【貨號(hào)】 BP-DL023

【規(guī)格】 7×50mL/7×100mL

【保存】 10~30℃，避光，12個(gè)月有效。

【產(chǎn)品組成】

  

【產(chǎn)品簡(jiǎn)介】

結(jié)締組織狹義上是指其含有的三種纖維：膠原纖維、網(wǎng)狀纖維、彈力纖維，而膠原纖維（collagenfiber）是分布最廣、含量最多的一種纖維。Masson三色染色又稱馬松染色，是結(jié)締組織染色中最經(jīng)典的一種方法，是膠原纖維染色權(quán)威而經(jīng)典的技術(shù)方法。所謂三色染色通常是指染胞核和能選擇性的顯示膠原纖維和肌纖維。該法染色原理不陰離子染料分子的大小和組織的滲透有關(guān)：分子的大小由分子量來(lái)體現(xiàn)，小分子量易穿透結(jié)構(gòu)致密、滲透性低的組織；而大分子量則只能進(jìn)入結(jié)構(gòu)疏松的、滲透性高的組織。然而，淡綠或苯胺藍(lán)的分子量都很大，因此Masson染色后肌纖維呈紅色，膠原纖維呈綠色（淡綠）或藍(lán)色（苯胺藍(lán)），主要用于區(qū)分膠原纖維和肌纖維。染色穩(wěn)定；分化時(shí)間短，1~2s；色彩清晰鮮艷；適用范圍廣，適宜于組織的石蠟切片、冰凍切片等染色；所染切片保存時(shí)間長(zhǎng)且不易褪色。

【使用方法】 

1、切片常規(guī)脫蠟至水，用配制好的Weigert鐵蘇木素染色5~10min。

2、用酸性乙醇分化液分化，水洗。

3、用Masson藍(lán)化液返藍(lán)，水洗。

4、蒸餾水洗1min。

5、麗春紅品紅染色液染色5~10min。

6、在上述操作過(guò)程中按蒸餾水:弱酸溶液=2:1比例配制弱酸工作液，用弱酸工作液洗1min。

7、磷鉬酸溶液洗1~2min。

8、用配制好的弱酸工作液洗1min。

9、直接入固綠染色液中染色1~2min。

10、用配制好的弱酸工作液洗1min。

11、95%乙醇快速脫水。無(wú)水乙醇脫水3次，每次5~10s。

12、二甲苯透明3次，每次1~2min。中性樹(shù)膠封固。

【染色結(jié)果】

  

【注意事項(xiàng)】

1、切片脫蠟應(yīng)盡量干凈。固定起著重要的作用，使用不同的固定液可延或縮短染色時(shí)間。

2、取A1、A2等量混合即為Weigert鐵蘇木素染液，一般24h失去染色力。

3、酸性乙醇分化時(shí)間應(yīng)根據(jù)切片厚薄、組織的類別和新舊而定。

4、弱酸溶液可使色彩更清晰鮮艷，如使用量大可自行配制0.1~0.3%乙酸溶液予以替代。

5、磷鉬酸分化時(shí)要在鏡下控制，分化到膠原纖維呈淡紅色、纖維呈紅色即可。分化時(shí)間根據(jù)染色深淺而定，一般1~2min。

6、Masson藍(lán)化液亦可自行配制Scott促藍(lán)液或0.1~1%碳酸鋰水溶液予以替代。

[1]  Ma X, Ma X, Ma Z, et al. The effects of uygur herb Hyssopus officinalis L. on the process of airway remodeling in asthmatic mice[J]. Evidence-Based Complementary and Alternative Medicine, 2014, 2014.

[2]  Wang W, Hu J, He C, et al. Heparinized PLLA/PLCL nanofibrous scaffold for potential engineering of small‐diameter blood vessel: Tunable elasticity and anticoagulation property[J]. Journal of Biomedical Materials Research Part A, 2015, 103(5): 1784-1797.

[3]  Wu C, Dong S, Li Y. Effects of miRNA-455 on cardiac hypertrophy induced by pressure overload[J]. International journal of molecular medicine, 2015, 35(4): 893-900.

[4]  Ren Z, Wang Y, Ma S, et al. Effective bone regeneration using thermosensitive poly (N-isopropylacrylamide) grafted gelatin as injectable carrier for bone mesenchymal stem cells[J]. ACS applied materials & interfaces, 2015, 7(34): 19006-19015.

[5]  Duan S, Yang X, Mei F, et al. Enhanced osteogenic differentiation of mesenchymal stem cells on poly (l‐lactide) nanofibrous scaffolds containing carbon nanomaterials[J]. Journal of Biomedical Materials Research Part A, 2015, 103(4): 1424-1435.

[6]  Ruan J, Wang X, Yu Z, et al. Enhanced Physiochemical and Mechanical Performance of Chitosan‐Grafted Graphene Oxide for Superior Osteoinductivity[J]. Advanced Functional Materials, 2016, 26(7): 1085-1097.

[7]  Jin J, Lv R, Guo J, et al. Improvement of left ventricular remodelling by inhibition of NF-κB in a rat model of myocardial infarction[J]. Heart, Lung and Circulation, 2016, 25(10): 1007-1012.

[8]  Jia Y, Wang W, Zhou X, et al. Synthesis and characterization of poly (glycerol sebacate)-based elastomeric copolyesters for tissue engineering applications[J]. Polymer Chemistry, 2016, 7(14): 2553-2564.

[9]  Xiao T, Luo J, Wu Z, et al. Effects of hydrogen sulfide on myocardial fibrosis and PI3K/AKT1-regulated autophagy in diabetic rats[J]. Molecular medicine reports, 2016, 13(2): 1765-1773.

[10]  Chi Y C, Shi C L, Zhou M, et al. Selective cyclooxygenase-2 inhibitor NS-398 attenuates myocardial fibrosis in mice after myocardial infarction via Snail signaling pathway[J]. Eur Rev Med Pharmacol Sci, 2017, 21(24): 5805-5812.

[11]  Ren Z, Ma S, Jin L, et al. Repairing a bone defect with a three-dimensional cellular construct composed of a multi-layered cell sheet on electrospun mesh[J]. Biofabrication, 2017, 9(2): 025036.

[12]  Liang B, Xiao T, Long J, et al. Hydrogen sulfide alleviates myocardial fibrosis in mice with alcoholic cardiomyopathy by downregulating autophagy[J]. International journal of molecular medicine, 2017, 40(6): 1781-1791.

[13]  Zhang Z, Li Z, Cao K, et al. Adjunctive therapy with statins reduces residual albuminuria/proteinuria and provides further renoprotection by downregulating the angiotensin II–AT1 pathway in hypertensive nephropathy[J]. Journal of hypertension, 2017, 35(7): 1442-1456.

[14]  Gong Z, Tang J, Xiang T, et al. Genome?wide identification of long noncoding RNAs in CCl4?induced liver fibrosis via RNA sequencing[J]. Molecular medicine reports, 2018, 18(1): 299-307.

[15]  Hou H T, Wang Y N, Shao S Z, et al. High calcium diet alleviates 5/6 nephrectomy-induced bone deteriorations of lumbar vertebrae in mice[J]. Experimental and therapeutic medicine, 2018, 15(4): 3483-3488.

[16]  Tang B, Zhang J G, Tan H Y, et al. Astragaloside IV inhibits ventricular remodeling and improves fatty acid utilization in rats with chronic heart failure[J]. Bioscience reports, 2018, 38(3).

[17]  Liu R, Zhang H B, Yang J, et al. Curcumin alleviates isoproterenol-induced cardiac hypertrophy and fibrosis through inhibition of autophagy and activation of mTOR[J]. European review for medical and pharmacological sciences, 2018, 22(21): 7500-7508.

[18]  Gong Z, Lin J, Zheng J, et al. Dahuang Zhechong pill attenuates CCl4‐induced rat liver fibrosis via the PI3K‐Akt signaling pathway[J]. Journal of cellular biochemistry, 2019.

[19]  Zhang W X, Tai G J, Li X X, et al. Inhibition of neointima hyperplasia by the combined therapy of linagliptin and metformin via AMPK/Nox4 signaling in diabetic rats[J]. Free Radical Biology and Medicine, 2019, 143: 153-163.

[20]  Deng Y, Guo S, Wei B, et al. Activation of nicotinic acetylcholine α7 receptor attenuates progression of monocrotaline-induced pulmonary hypertension in Rats by downregulating the NLRP3 inflammasome[J]. Frontiers in Pharmacology, 2019, 10: 128.

[21]  Lin J, Deng C, Peng Y, et al. Dynamic Changes in MMP1 and TIMP1 in the Antifibrotic Process of Dahuang Zhechong Pill in Rats with Liver Fibrosis[J]. Open Chemistry, 2019, 17(1): 346-356.

[22]  Yuan Z, Wei P, Huang Y, et al. Injectable PLGA microspheres with tunable magnesium ion release for promoting bone regeneration[J]. Acta biomaterialia, 2019, 85: 294-309.

[23]  Wei P, Jing W, Yuan Z, et al. Vancomycin-and Strontium-Loaded Microspheres with Multifunctional Activities against Bacteria, in Angiogenesis, and in Osteogenesis for Enhancing Infected Bone Regeneration[J]. ACS applied materials & interfaces, 2019, 11(34): 30596-30609.

[24]  Wei P, Yuan Z, Jing W, et al. Strengthening the potential of biomineralized microspheres in enhancing osteogenesis via incorporating alendronate[J]. Chemical Engineering Journal, 2019, 368: 577-588.

[25]  Zhou C, Li C, Wang Q, et al. Histopathological and proteomic analyses identify integrin-β1 as a potential mediator of phlebosclerosis in uremic patients[J]. Clinical and Experimental Nephrology, 2019: 1-9.

[26]  Shi A, Heinayati A, Bao D, et al. Small molecule inhibitor of TGF-β signaling enables robust osteogenesis of autologous GMSCs to successfully repair minipig severe maxillofacial bone defects[J]. Stem cell research & therapy, 2019, 10(1): 172.successfully repair minipig severe maxillofacial bone defects[J]. Stem cell research & therapy, 2019, 10(1): 172.